As molecular methods like PCR continue to reshape microbiological testing, the preparation of cannabis samples for molecular quantification of tragets like total yeast and mold (TYM) and pathogens is critical to ensuring accurate, reliable results. This session will explore best practices for sample preparation, including homogenization techniques, enrichment strategies, and extraction methods that optimize sensitivity and specificity. We will discuss challenges unique to cannabis matrices, such as sample inhibition and variability, and how proper preparation can enhance detection while maintaining regulatory compliance. Attendees will gain insights into refining molecular workflows to improve both efficiency and data integrity in cannabis microbiology testing.